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Anal Biochem. 2009 Sep 15;392(2):183-5. doi: 10.1016/j.ab.2009.05.032. Epub 2009 May 27.

Enzymatic assay of D-glucuronate using uronate dehydrogenase.

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Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.


D-Glucuronate is a key metabolite in the process of detoxification of xenobiotics and in a recently constructed synthetic pathway to produce D-glucaric acid, a "top value-added chemical" from biomass. A simple and specific assay of D-glucuronate would be useful for studying these processes, but existing assays are either time-consuming or nonspecific. Using uronate dehydrogenase cloned from Agrobacterium tumefaciens, we developed an assay for D-glucuronate with a detection limit of 5 microM. This method was shown to be more suitable for a system with many interfering compounds than previous methods and was also applied to assays for myo-inositol oxygenase activity.

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