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Biotechniques. 2009 May;46(6):409-19. doi: 10.2144/000113168.

Analysis of insoluble proteins.

Author information

1
Department of Chemistry, Wayne State University, Department of Chemistry, Detroit, Michigan, USA.

Abstract

The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20-30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time-consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.

PMID:
19480635
DOI:
10.2144/000113168
[Indexed for MEDLINE]
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