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PLoS One. 2009 May 29;4(5):e5744. doi: 10.1371/journal.pone.0005744.

Lifeact-mEGFP reveals a dynamic apical F-actin network in tip growing plant cells.

Author information

1
Department of Biology, University of Massachusetts, Amherst, Massachusetts, United States of America.

Abstract

BACKGROUND:

Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.

METHODOLOGY/PRINCIPAL FINDINGS:

In this report we use Lifeact-mEGFP, an actin probe that does not affect the dynamics of actin, to visualize actin in the moss Physcomitrella patens and pollen tubes from Lilium formosanum and Nicotiana tobaccum. Lifeact-mEGFP robustly labels actin microfilaments, particularly in the apex, in both moss protonemata and pollen tubes. Lifeact-mEGFP also labels filamentous actin structures in other moss cell types, including cells of the gametophore.

CONCLUSIONS/SIGNIFICANCE:

Lifeact-mEGFP, when expressed at optimal levels does not alter moss protonemal or pollen tube growth. We suggest that Lifeact-mEGFP represents an exciting new versatile probe for further studies of actin's role in tip growing plant cells.

PMID:
19478943
PMCID:
PMC2684639
DOI:
10.1371/journal.pone.0005744
[Indexed for MEDLINE]
Free PMC Article

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