Format

Send to

Choose Destination
Nat Methods. 2009 Jun;6(6):435-7. doi: 10.1038/nmeth.1334.

A toolkit for high-throughput, cross-species gene engineering in Drosophila.

Author information

1
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Abstract

We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.

PMID:
19465918
DOI:
10.1038/nmeth.1334
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center