Format

Send to

Choose Destination
See comment in PubMed Commons below

[Development of a multiplex PCR-suspension array for simultaneous detection of five bioterrorism bacteria].

[Article in Chinese]

Author information

  • 1China Academy of Inspection and Quarantin, Beijing 100025, China.

Abstract

OBJECTIVE:

To develop a rapid, high-throughput screening method of gene suspension array technique to simultaneously detect five bioterrorism bacteria: Bacillus anthracis, Francisella tularensis, Yersinia pestis, Brucella spp. and Burkholderia pseudomallei.

METHODS:

Highly validated specific primers were used to amplify diagnostic regions unique to each pathogen. Biotin labelled PCR products were hybridized to corresponding probes coupling on the unique sets of fluorescent beads. The hybridized beads were processed through the Bio-plex, which identified the presence of PCR products.

RESULTS:

Multiplex PCR-suspension array can detect five bioterrorism bacteria correctly with high specificity and high sensitivity, the results suggest the utility of suspension array system for high-throughput screening of bioterrorism samples.

CONCLUSION:

A multiplex PCR-suspension array for rapid detection of five bioterrorism bacteria was established.

PMID:
19462919
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center