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Plant Physiol. 2009 Jul;150(3):1482-93. doi: 10.1104/pp.109.140269. Epub 2009 May 20.

CDKB1;1 forms a functional complex with CYCA2;3 to suppress endocycle onset.

Author information

1
Department of Plant Systems Biology, Flanders Institute for Biotechnology, 9052 Ghent, Belgium.

Abstract

The mitosis-to-endocycle transition requires the controlled inactivation of M phase-associated cyclin-dependent kinase (CDK) activity. Previously, the B-type CDKB1;1 was identified as an important negative regulator of endocycle onset. Here, we demonstrate that CDKB1;1 copurifies and associates with the A2-type cyclin CYCA2;3. Coexpression of CYCA2;3 with CDKB1;1 triggered ectopic cell divisions and inhibited endoreduplication. Moreover, the enhanced endoreduplication phenotype observed after overexpression of a dominant-negative allele of CDKB1;1 could be partially complemented by CYCA2;3 co-overexpression, illustrating that both subunits unite in vivo to form a functional complex. CYCA2;3 protein stability was found to be controlled by CCS52A1, an activator of the anaphase-promoting complex. We conclude that CCS52A1 participates in endocycle onset by down-regulating CDKB1;1 activity through the destruction of CYCA2;3.

PMID:
19458112
PMCID:
PMC2705057
DOI:
10.1104/pp.109.140269
[Indexed for MEDLINE]
Free PMC Article

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