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Anal Biochem. 2009 Aug 15;391(2):151-3. doi: 10.1016/j.ab.2009.05.015. Epub 2009 May 18.

Rapid and high-throughput analysis of N-glycans from ovarian cancer serum using a 96-well plate platform.

Author information

1
Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea.

Abstract

We present a rapid and high-throughput human serum N-glycan preparation technology using 96-well plate-based procedures. The released N-glycans from polyvinylidene fluoride (PVDF) membrane filter plate are subsequently loaded to porous graphitic carbon (PGC) containing a 96-well plate to remove salts and other contaminants without sacrificing accuracy or reproducibility. This robust glycan preparation technology is applied to ovarian cancer diagnosis using 5 microl of patient serum.

PMID:
19457428
DOI:
10.1016/j.ab.2009.05.015
[Indexed for MEDLINE]

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