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J Am Chem Soc. 2009 May 27;131(20):6952-4. doi: 10.1021/ja900186n.

Atomic-level mapping of antibody epitopes on a GPCR.

Author information

1
Integral Molecular, Inc., 3701 Market Street, Fourth Floor, Philadelphia, Pennsylvania 19104, USA.

Abstract

Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.

PMID:
19453194
PMCID:
PMC2943208
DOI:
10.1021/ja900186n
[Indexed for MEDLINE]
Free PMC Article

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