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Biochem Biophys Res Commun. 2009 Jul 31;385(3):307-13. doi: 10.1016/j.bbrc.2009.05.048. Epub 2009 May 18.

Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer.

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1
Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taiwan.

Abstract

Aldehyde dehydrogenase 1 (ALDH1) has been considered to be a marker for cancer stem cells. However, the role of ALDH1 in head and neck squamous cell carcinoma (HNSCC) has yet to be determined. In this study, we isolated ALDH1-positive cells from HNSCC patients and showed that these HNSCC-ALDH1+ cells displayed radioresistance and represented a reservoir for generating tumors. Based on microarray findings, the results of Western blotting and immunofluorescent assays further confirmed that ALDH1+-lineage cells showed evidence of having epithelial-mesenchymal transition (EMT) shifting and endogenously co-expressed Snail. Furthermore, the knockdown of Snail expression significantly decreased the expression of ALDH1, inhibited cancer stem-like properties, and blocked the tumorigenic abilities of CD44+CD24(-)ALDH1+ cells. Finally, in a xenotransplanted tumorigenicity study, we confirmed that the treatment effect of chemoradiotherapy for ALDH1+ could be improved by Snail siRNA. In summary, it is likely that ALDH1 is a specific marker for the cancer stem-like cells of HNSCC.

PMID:
19450560
DOI:
10.1016/j.bbrc.2009.05.048
[Indexed for MEDLINE]
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