Biodegradation of brominated aromatics by cultures and laccase of Trametes versicolor

Chemosphere. 2009 Aug;76(6):826-32. doi: 10.1016/j.chemosphere.2009.04.016. Epub 2009 May 13.

Abstract

2-Bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3), 2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobisphenol A (6) (1 mM each) added to growing submerged cultures of Trametes versicolor CCBAS 612 were eliminated by 65-85% from the culture medium within 4d. Extracellular laccase activity in the culture medium was influenced by the type of brominated compound added. Maximum level of laccase (63 U L(-1)) was found in the culture with 2-bromophenol. Tetrabromobisphenol A was degraded by a commercial laccase from Trametes versicolor in absence of any oxidation mediator, hydroxylated dibrominated compounds being detected as soluble reaction products by LC/MS. A significant degradation of brominated phenols by laccase was achieved only in the presence of ABTS structural characterization of major products suggesting reaction between bromophenol and ABTS radicals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Hydrocarbons, Brominated / chemistry
  • Hydrocarbons, Brominated / metabolism*
  • Laccase / metabolism*
  • Phenols / chemistry
  • Phenols / metabolism*
  • Polybrominated Biphenyls / metabolism
  • Trametes / enzymology*
  • Water Pollutants, Chemical / chemistry
  • Water Pollutants, Chemical / metabolism*

Substances

  • Hydrocarbons, Brominated
  • Phenols
  • Polybrominated Biphenyls
  • Water Pollutants, Chemical
  • 2,6-dibromophenol
  • 2-bromophenol
  • Laccase
  • tetrabromobisphenol A
  • 2,4-dibromophenol
  • 4-bromophenol
  • 2,4,6-tribromophenol