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BMC Bioinformatics. 2009 May 14;10:145. doi: 10.1186/1471-2105-10-145.

flowClust: a Bioconductor package for automated gating of flow cytometry data.

Author information

1
Department of Statistics, University of British Columbia, 333-6356 Agricultural Road, Vancouver, BC, V6T1Z2, Canada. c.lo@stat.ubc.ca

Abstract

BACKGROUND:

As a high-throughput technology that offers rapid quantification of multidimensional characteristics for millions of cells, flow cytometry (FCM) is widely used in health research, medical diagnosis and treatment, and vaccine development. Nevertheless, there is an increasing concern about the lack of appropriate software tools to provide an automated analysis platform to parallelize the high-throughput data-generation platform. Currently, to a large extent, FCM data analysis relies on the manual selection of sequential regions in 2-D graphical projections to extract the cell populations of interest. This is a time-consuming task that ignores the high-dimensionality of FCM data.

RESULTS:

In view of the aforementioned issues, we have developed an R package called flowClust to automate FCM analysis. flowClust implements a robust model-based clustering approach based on multivariate t mixture models with the Box-Cox transformation. The package provides the functionality to identify cell populations whilst simultaneously handling the commonly encountered issues of outlier identification and data transformation. It offers various tools to summarize and visualize a wealth of features of the clustering results. In addition, to ensure its convenience of use, flowClust has been adapted for the current FCM data format, and integrated with existing Bioconductor packages dedicated to FCM analysis.

CONCLUSION:

flowClust addresses the issue of a dearth of software that helps automate FCM analysis with a sound theoretical foundation. It tends to give reproducible results, and helps reduce the significant subjectivity and human time cost encountered in FCM analysis. The package contributes to the cytometry community by offering an efficient, automated analysis platform which facilitates the active, ongoing technological advancement.

PMID:
19442304
PMCID:
PMC2701419
DOI:
10.1186/1471-2105-10-145
[Indexed for MEDLINE]
Free PMC Article

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