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PLoS One. 2009;4(5):e5590. doi: 10.1371/journal.pone.0005590. Epub 2009 May 18.

Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.

Author information

1
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany.

Abstract

BACKGROUND:

T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans.

METHODOLOGY/PRINCIPAL FINDINGS:

We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI.

CONCLUSIONS:

Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI.

PMID:
19440342
PMCID:
PMC2680040
DOI:
10.1371/journal.pone.0005590
[Indexed for MEDLINE]
Free PMC Article

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