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Mol Cell Biol. 2009 Jul;29(14):4014-23. doi: 10.1128/MCB.01582-08. Epub 2009 May 11.

Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation.

Author information

1
Department of Biochemistry and Howard Hughes Medical Institute, University of Utah School of Medicine, 15 N Medical Drive East, Room 4800, Salt Lake City, UT 84112-5650, USA. markparnell1@gmail.com

Abstract

The biogenesis of the large (60S) ribosomal subunit in eukaryotes involves nucleolar, nucleoplasmic, and cytoplasmic steps. The cytoplasmic protein Rei1, found in all eukaryotes, was previously shown to be necessary for the nuclear reimport of 60S subunit export factor Arx1. In this study we investigate the function of Reh1, a protein with high sequence similarity to Rei1. We demonstrate an overlapping function for Reh1 and Rei1 in the cytoplasmic maturation of the 60S subunit that is independent of Arx1 recycling. We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form.

PMID:
19433447
PMCID:
PMC2704747
DOI:
10.1128/MCB.01582-08
[Indexed for MEDLINE]
Free PMC Article
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