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J Infect Dis. 2009 Jun 1;199(11):1671-9. doi: 10.1086/598966.

Experimental infection of human volunteers with Haemophilus ducreyi: fifteen years of clinical data and experience.

Author information

1
Department of Medicine, Indiana University School of Medicine, Indianapolis, USA. dmjanowi@iupui.edu

Abstract

Haemophilus ducreyi causes chancroid, which facilitates transmission of human immunodeficiency virus type 1. To better understand the biology of H. ducreyi, we developed a human inoculation model. In the present article, we describe clinical outcomes for 267 volunteers who were infected with H. ducreyi. There was a relationship between papule formation and estimated delivered dose. The outcome (either pustule formation or resolution) of infected sites for a given subject was not independent; the most important determinants of pustule formation were sex and host effects. When 41 subjects were infected a second time, their outcomes segregated toward their initial outcome, confirming the host effect. Subjects with pustules developed local symptoms that required withdrawal from the study after a mean of 8.6 days. There were 191 volunteers who had tissue biopsy performed, 173 of whom were available for follow-up analysis; 28 (16.2%) of these developed hypertrophic scars, but the model was otherwise safe. Mutant-parent trials confirmed key features in H. ducreyi pathogenesis, and the model has provided an opportunity to study differential human susceptibility to a bacterial infection.

PMID:
19432549
PMCID:
PMC2682218
DOI:
10.1086/598966
[Indexed for MEDLINE]
Free PMC Article

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