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Inflamm Res. 2009 Nov;58(11):791-6. doi: 10.1007/s00011-009-0049-z. Epub 2009 May 9.

LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts.

Author information

1
Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden.

Abstract

OBJECTIVE AND DESIGN:

Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROalpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROalpha by oral fibroblasts have not previously been presented.

MATERIALS AND METHODS:

GROalpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA.

RESULTS:

We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROalpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROalpha and GROalpha protein. The steroid hormone estrogen had no effect on LPS-induced GROalpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROalpha production, and the NF-kappaB blocker MG 132 fully prevented LPS-induced GROalpha.

CONCLUSIONS:

Oral fibroblasts respond to LPS stimulation by increasing GROalpha production via the transcription factor NF-kappaB, suggesting that this mechanism may be involved in development of periodontal inflammation.

PMID:
19430878
DOI:
10.1007/s00011-009-0049-z
[Indexed for MEDLINE]

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