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Nat Methods. 2009 Jun;6(6):439-41. doi: 10.1038/nmeth.1329. Epub 2009 May 10.

TU-tagging: cell type-specific RNA isolation from intact complex tissues.

Author information

1
Institute of Neuroscience, Howard Hughes Medical Institute, University of Oregon, Eugene, Oregon, USA.

Abstract

We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.

PMID:
19430475
PMCID:
PMC2783170
DOI:
10.1038/nmeth.1329
[Indexed for MEDLINE]
Free PMC Article

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