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J Neurosci Methods. 2009 May 15;179(2):184-90. doi: 10.1016/j.jneumeth.2009.01.024. Epub 2009 Feb 4.

A novel system allowing long-term simultaneous video-electroencephalography recording, drug infusion and blood sampling in rats.

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1
The Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Melbourne, Victoria, Australia.

Abstract

INTRODUCTION:

Electroencephalography (EEG) recording and drug administration is commonly used for neurological experiments in rats, but is typically cumbersome due the use of multiple lines. We have developed a unique system, which allows long-term simultaneous video-electroencephalography recording, drug infusion and blood sampling in rats.

METHODS:

The vEEG/drug infusion system was designed and tested on two contrasting animal models of epilepsy. Animals were implanted with EEG-electrodes and a jugular vein cannula fixed in a head cap, avoiding an additional cable for tethering. In an acute infusion study (n=16), repeated blood samples were taken after i.v. bolus injection of valproate. In a subset of these rats (n=10), paired blood samples were removed from the jugular vein and the heart after valproate administration. In a chronic infusion study (n=38), heparinised (4IU/h) saline or valproate (42mg/kg/h) was infused continuously for up to 17 days.

RESULTS:

In the acute study, repeated blood samples showed a decrease in plasma valproate levels over time following bolus injection. In the chronic study, high quality continuous EEG was achieved and 79% of animals were successfully infused throughout the planned infusion period (13-17 days), with 66% of projected blood samples able to be taken during the infusion. There was a high correlation between the jugular vein and cardiac plasma levels of valproate (Spearman test, r=0.69; p<0.05).

CONCLUSION:

This system is ideal for pharmacokinetic/dynamic studies and long-term drug infusion where simultaneous EEG and/or frequent blood sampling are desired.

PMID:
19428526
DOI:
10.1016/j.jneumeth.2009.01.024
[Indexed for MEDLINE]
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