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J Neurosci Methods. 2009 May 15;179(2):166-72. doi: 10.1016/j.jneumeth.2009.01.019. Epub 2009 Jan 31.

Selective labeling of retinal ganglion cells with calcium indicators by retrograde loading in vitro.

Author information

1
Dept. of Electrical Engineering, University of Southern California, Los Angeles, CA 90033, USA.

Abstract

Here we present a retrograde loading technique that makes it possible for the first time to rapidly load a calcium indicator in the majority of retinal ganglion cells (RGCs) in salamander retina, and then to observe physiological activity of these dye-loaded cells. Dextran-conjugated calcium indicator, dissolved in water, was applied to the optic nerve stump. Following dye loading, the isolated retina was mounted on a microelectrode array to demonstrate that electrical activity and calcium activity were preserved, as the retina responded to electrical stimuli.

PMID:
19428523
PMCID:
PMC3345189
DOI:
10.1016/j.jneumeth.2009.01.019
[Indexed for MEDLINE]
Free PMC Article

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