Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2009 Jun;6(6):447-50. doi: 10.1038/nmeth.1326. Epub 2009 May 3.

Automated unrestricted multigene recombineering for multiprotein complex production.

Author information

1
European Molecular Biology Laboratory, Grenoble Outstation, Grenoble, France.

Abstract

Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.

PMID:
19412171
DOI:
10.1038/nmeth.1326
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center