Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion

Surg Today. 2009;39(5):407-13. doi: 10.1007/s00595-008-3900-x. Epub 2009 Apr 30.

Abstract

Purpose: We investigated whether Tempol, a water-soluble antioxidant, prevents the harmful effects of superior mesenteric ischemia/reperfusion on intestinal tissues in rats.

Methods: The rats were divided into three groups of 10. In group 1, the superior mesenteric artery (SMA) was isolated but not occluded, and in groups 2 and 3 the superior mesenteric artery was occluded for 60 min. After that, the clamp was removed and reperfusion began. In group 3, 5 min before the start of reperfusion, a bolus dose of 30 mg/kg Tempol was administered intravenously and continued at a dose of 30 mg/kg for 60 min. All animals were euthanized after 24 h and tissue samples were collected for analysis.

Results: There was a significant increase in myeloperoxidase activity, malondialdehyde levels, and the incidence of bacterial translocation in group 2, with a decrease in glutathione levels. These parameters were found to be normalized in group 3. The intestinal mucosal injury score in group 2 was significantly higher than those in groups 1 and 3.

Conclusion: Tempol prevents bacterial translocation while precluding the harmful effects of ischemia/reperfusion injury on intestinal tissues in a rat model of superior mesenteric artery occlusion.

MeSH terms

  • Acute Disease
  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology*
  • Arterial Occlusive Diseases / pathology*
  • Bacterial Translocation / drug effects*
  • Cyclic N-Oxides / administration & dosage
  • Cyclic N-Oxides / pharmacology*
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / pathology*
  • Lipid Peroxidation / drug effects
  • Male
  • Malondialdehyde / metabolism
  • Mesenteric Arteries / drug effects
  • Mesenteric Arteries / pathology*
  • Models, Animal
  • Myocardial Ischemia / complications*
  • Myocardial Ischemia / prevention & control
  • Myocardial Reperfusion / adverse effects*
  • Neutrophils / drug effects
  • Neutrophils / enzymology
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species
  • Spin Labels

Substances

  • Antioxidants
  • Cyclic N-Oxides
  • Reactive Oxygen Species
  • Spin Labels
  • Malondialdehyde
  • tempol