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Int J Radiat Biol. 2009 Apr;85(4):369-76. doi: 10.1080/09553000902781147.

The response of gamma-H2AX in human lymphocytes and lymphocytes subsets measured in whole blood cultures.

Author information

1
Department of Physics, Carleton University, Ottawa, ON, Canada.

Abstract

PURPOSE:

To assess the use of phosphorylated histone H2AX protein (gamma-H2AX) in human blood leukocytes as a rapid screening tool for radiation biodosimetry using a method that examines the characteristics of gamma-H2AX phosphorylation in a variety of lymphocyte subsets following exposure to radiation.

MATERIALS AND METHODS:

Human peripheral blood exposed to 0-10 Gy of (137)Cs irradiation and cultured for 0-48 h was analysed using a rapid whole blood flow cytometry assay to measure gamma-H2AX phosphorylation in different lymphocyte subpopulations.

RESULTS:

Lymphocyte subsets displayed a similar linear dose response relationship, although cluster of differentiation 4(+) (CD4(+)) and CD8(+) lymphocytes were found to express H2AX phosphorylation on the order of 1.5 times higher than CD19(+) lymphocytes. Phosphorylation of all lymphocyte subsets reached a maximum at 1.5 h and had essentially returned to baseline levels 24 h post-exposure.

CONCLUSIONS:

Differences in the expression level of gamma-H2AX between lymphocyte subsets were minimal. The usefulness of this assay for radiation biodosimetry is hampered by its relatively quick lifetime kinetics and large inter-individual variation. Therefore, it could only be useful if samples were obtained within 24 h of exposure. Even in this situation, the assay could only be used as an indicator of exposure and not a dosimeter.

PMID:
19399682
DOI:
10.1080/09553000902781147
[Indexed for MEDLINE]

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