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PLoS One. 2009;4(4):e1. doi: 10.1371/journal.pone.0005361. Epub 2009 Apr 28.

Betulin is a potent anti-tumor agent that is enhanced by cholesterol.

Author information

1
Laboratory for Experimental Oncology and Radiobiology (LEXOR), Center for Experimental and Molecular Medicine, Academic Medical Center (AMC), Amsterdam, The Netherlands.

Abstract

Betulinic Acid (BetA) and its derivatives have been extensively studied in the past for their anti-tumor effects, but relatively little is known about its precursor Betulin (BE). We found that BE induces apoptosis utilizing a similar mechanism as BetA and is prevented by cyclosporin A (CsA). BE induces cell death more rapidly as compared to BetA, but to achieve similar amounts of cell death a considerably higher concentration of BE is needed. Interestingly, we observed that cholesterol sensitized cells to BE-induced apoptosis, while there was no effect of cholesterol when combined with BetA. Despite the significantly enhanced cytotoxicity, the mode of cell death was not changed as CsA completely abrogated cell death. These results indicate that BE has potent anti-tumor activity especially in combination with cholesterol.

PMID:
19399186
PMCID:
PMC2671171
DOI:
10.1371/journal.pone.0005361
[Indexed for MEDLINE]
Free PMC Article

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