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J Cell Biol. 2009 May 4;185(3):397-407. doi: 10.1083/jcb.200903088. Epub 2009 Apr 27.

Active establishment of centromeric CENP-A chromatin by RSF complex.

Author information

1
Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi, Japan.

Abstract

Centromeres are chromosomal structures required for equal DNA segregation to daughter cells, comprising specialized nucleosomes containing centromere protein A (CENP-A) histone, which provide the basis for centromeric chromatin assembly. Discovery of centromere protein components is progressing, but knowledge related to their establishment and maintenance remains limited. Previously, using anti-CENP-A native chromatin immunoprecipitation, we isolated the interphase-centromere complex (ICEN). Among ICEN components, subunits of the remodeling and spacing factor (RSF) complex, Rsf-1 and SNF2h proteins, were found. This paper describes the relationship of the RSF complex to centromere structure and function, demonstrating its requirement for maintenance of CENP-A at the centromeric core chromatin in HeLa cells. The RSF complex interacted with CENP-A chromatin in mid-G1. Rsf-1 depletion induced loss of centromeric CENP-A, and purified RSF complex reconstituted and spaced CENP-A nucleosomes in vitro. From these data, we propose the RSF complex as a new factor actively supporting the assembly of CENP-A chromatin.

PMID:
19398759
PMCID:
PMC2700388
DOI:
10.1083/jcb.200903088
[Indexed for MEDLINE]
Free PMC Article
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