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Methods. 2009 Aug;48(4):375-80. doi: 10.1016/j.ymeth.2009.04.006. Epub 2009 May 3.

Genetic analysis of P-TEFb function via heterologous nucleic acid tethering systems.

Author information

1
Departments of Medicine, Microbiology and Immunology, Rosalind Russell Medical Research Center, University of California San Francisco, San Francisco, CA 94143, USA.

Abstract

Recent global genetic analyses demonstrated that the regulation of gene expression at the level of transcription elongation is a common feature in eukaryotes. The positive transcription elongation factor P-TEFb plays a critical role in this process. P-TEFb is a cyclin-dependent kinase, which controls the fraction of RNA polymerase II (RNAP II) that can enter productive elongation. While the biochemical properties of P-TEFb and its associated factors have been characterized extensively in vitro, its function in vivo remains less well understood. In this paper, we describe various heterologous nucleic acid tethering systems that can be used to examine transcription factors that function via P-TEFb.

PMID:
19398004
PMCID:
PMC2745987
DOI:
10.1016/j.ymeth.2009.04.006
[Indexed for MEDLINE]
Free PMC Article

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