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J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 15;877(14-15):1575-82. doi: 10.1016/j.jchromb.2009.04.003. Epub 2009 Apr 7.

A modified method for the determination of tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human urine by solid phase extraction using a molecularly imprinted polymer and liquid chromatography tandem mass spectrometry.

Author information

1
Department of Pharmaceutics, Virginia Commonwealth University, Richmond, VA 23298, USA.

Abstract

The work described in this paper represents an improvement over a previously published method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in human urine by solid phase extraction on a molecularly imprinted polymer column coupled with HPLC and -MS/MS detection. The influence of ion suppression due to sample matrix effect was evaluated, and found to influence the response of NNAL. By changing the liquid chromatography conditions, the response for this method was enhanced approximately 25-fold through avoidance of ionization suppression that was found with a previously published method and sample throughput has been improved. The dynamic range of the assay extends from 20 to 2500 pg/mL with a mean r(2)>0.998. The lower limit of quantitation for the assay was 20 pg/mL despite the use of an inherently lower sensitivity instrument. The method was validated according to current FDA guidelines for bioanalytical method validations.

PMID:
19395323
DOI:
10.1016/j.jchromb.2009.04.003
[Indexed for MEDLINE]

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