Format

Send to

Choose Destination
J Microbiol Methods. 2009 Jul;78(1):97-100. doi: 10.1016/j.mimet.2009.04.007. Epub 2009 Apr 23.

An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins.

Author information

1
Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA.

Abstract

We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h . It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C. difficile toxins in the fecal and serum specimens of experimentally infected piglets.

PMID:
19393695
PMCID:
PMC2740367
DOI:
10.1016/j.mimet.2009.04.007
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center