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Ann Nutr Metab. 2009;54(2):151-7. doi: 10.1159/000214834. Epub 2009 Apr 22.

Green tea (-)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice.

Author information

1
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, Korea.

Abstract

AIMS:

The aim of this study was to investigate the antiobesity effect of (-)-epigallocatechin-3-gallate (EGCG) in diet-induced obese mice.

METHODS:

Male C57BL/6J mice were fed on a high-fat diet for 8 weeks to induce obesity. Subsequently they were divided into 3 groups and were maintained on a high-fat control diet or high-fat diets supplemented with 0.2 or 0.5% EGCG (w/w) for a further 8 weeks. Changes in the expression of genes related to lipid metabolism and fatty acid oxidation were analyzed in white adipose tissue, together with biometric and blood parameters.

RESULTS:

Experimental diets supplemented with EGCG resulted in reduction of body weight and mass of various adipose tissues in a dose-dependent manner. EGCG diet also considerably lowered the levels of plasma triglyceride and liver lipid. In the epididymal white adipose tissue of EGCG diet-fed mice, the mRNA levels of adipogenic genes such as peroxisome proliferator-activated receptor-gamma (PPAR-gamma), CCAAT enhancer-binding protein-alpha (C/EBP-alpha), regulatory element-binding protein-1c (SREBP-1c), adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL) and fatty acid synthase (FAS) were significantly decreased. However, the mRNA levels of carnitine palmitoyl transferase-1 (CPT-1) and uncoupling protein 2 (UCP2), as well as lipolytic genes such as hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), were significantly increased.

CONCLUSION:

These results suggest that green tea EGCG effectively reduces adipose tissue mass and ameliorates plasma lipid profiles in high-fat diet-induced obese mice. These effects might be at least partially mediated via regulation of the expression of multiple genes involved in adipogenesis, lipolysis, beta-oxidation and thermogenesis in white adipose tissue.

PMID:
19390166
DOI:
10.1159/000214834
[Indexed for MEDLINE]

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