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Wei Sheng Wu Xue Bao. 2009 Jan;49(1):117-22.

[Effect of exopolysaccharide from Bifidobacterium bifidum on cell of gastric cancer and human telomerase reverse transcriptase].

[Article in Chinese]

Author information

1
Key Laboratory of Dairy Science, Ministry of Education, China. chenxu19800203@126.com

Abstract

OBJECTIVE:

To study the inhibition of human gastric cancer cell line BGC-823 by exopolysaccharide (B. EPS) extracted from Bifidobacterium bifidum and the effect on the activity of telomerase rate-limiting factor human telomerase reverse transcriptase (hTERT).

METHODS:

In vitro human gastric cancer cell line BGC-823 were treated by three concentrations of B. EPS. The inhibition rate of gastric cancer cell line BGC-823, the morphology changes of cells and the apoptosis in the initial stage of cell was observed respectively by MTF, inverted fluorescent microscope and the Annexin V-FITC/PI flow cytometry. In addition, the effect of B. EPS on cell telomerase rate-limiting factor hTERT mRNA was tested by RT-PCR; the changes in the concentration of calcium ions inside the cytoplasm were detected by spectrofluorometer.

RESULTS:

B. EPS inhibited the growth of gastric cancer cell BGC-823 (P < 0.05), the inhibition rate increased and the concentration of B.EPS showed dose-time response relation. The expression of cell telomerase rate-limiting factor hTERT mRNA decrease after effected by B.EPS (P < 0.05), and showed dose-effect relation with B. EPS. The concentration of calcium ion in cytoplasm was higher than the control apparently (P < 0.05).

CONCLUSION:

The apoptosis mechanism of human gastric cancer cell line BGC-823 induced by B. EPS might be relevant with the changes of the hTERT mRNA expression and the calcium ions concentration in cytoplasm.

PMID:
19388274
[Indexed for MEDLINE]

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