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J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 15;877(28):3300-8. doi: 10.1016/j.jchromb.2009.03.038. Epub 2009 Mar 31.

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection.

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Institute of Chemistry, Military University of Technology, Warsaw, Poland.


Analysis of urine for endogenous thiols and thiol drugs content by HPLC with ultraviolet detection is addressed. Other methodologies for detection and determination of thiols in urine are only mentioned. Outline of metabolism, role of main biological thiols in physiological and pathological processes and their reference concentrations in urine are presented. In particular, urine sample preparation procedures, including reduction of thiol disulfides, chemical derivatization and reversed-phase HPLC separation steps are discussed. Some experimental details of analytical procedures for determination of endogenous thiols cysteine, cysteinylglycine, homocysteine, N-acetylcysteine, thioglycolic acid; and thiol drugs cysteamine, tiopronin, D-penicillamine, captopril, mesna, methimazole, propylthiouracil and thioguanine are reviewed.

[Indexed for MEDLINE]

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