Format

Send to

Choose Destination
BMC Genomics. 2009 Apr 21;10:168. doi: 10.1186/1471-2164-10-168.

The gamma-gliadin multigene family in common wheat (Triticum aestivum) and its closely related species.

Author information

1
Triticeae Research Institute, Sichuan Agricultural University, Yaan, Sichuan 625014, PR China. pengfeiqi@hotmail.com

Abstract

BACKGROUND:

The unique properties of wheat flour primarily depend on gluten, which is the most important source of protein for human being. gamma-Gliadins have been considered to be the most ancient of the wheat gluten family. The complex family structure of gamma-gliadins complicates the determination of their function. Moreover, gamma-gliadins contain several sets of celiac disease epitopes. However, no systematic research has been conducted yet.

RESULTS:

A total of 170 gamma-gliadin genes were isolated from common wheat and its closely related species, among which 138 sequences are putatively functional. The ORF lengths of these sequences range from 678 to 1089 bp, and the repetitive region is mainly responsible for the size heterogeneity of gamma-gliadins. The repeat motif P(Q/L/S/T/I/V/R/A)F(S/Y/V/Q/I/C/L)P(R/L/S/T/H/C/Y)Q1-2(P(S/L/T/A/F/H)QQ)1-2is repeated from 7 to 22 times. Sequence polymorphism and linkage disequilibrium analyses show that gamma-gliadins are highly diverse. Phylogenic analyses indicate that there is no obvious discrimination between Sitopsis and Ae. tauschii at the Gli-1 loci, compared with diploid wheat. According to the number and placement of cysteine residues, we defined nine cysteine patterns and 17 subgroups. Alternatively, we classified gamma-gliadins into two types based on the length of repetitive domain. Amino acid composition analyses indicate that there is a wide range of essential amino acids in gamma-gliadins, and those gamma-gliadins from subgroup SG-10 and SG-12 and gamma-gliadins with a short repetitive domain are more nutritional. A screening of toxic epitopes shows that gamma-gliadins with a pattern of C9 and gamma-gliadins with a short repetitive domain almost lack any epitopes.

CONCLUSION:

gamma-Gliadin sequences in wheat and closely related Aegilops species are diverse. Each group/subgroup contributes differently to nutritional quality and epitope content. It is suggested that the genes with a short repetitive domain are more nutritional and valuable. Therefore, it is possible to breed wheat varieties, the gamma-gliadins of which are less, even non-toxic and more nutritional.

PMID:
19383144
PMCID:
PMC2685405
DOI:
10.1186/1471-2164-10-168
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center