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J Proteome Res. 2009 Jul;8(7):3265-77. doi: 10.1021/pr800873q.

Characterization of human skeletal muscle biopsy samples using shotgun proteomics.

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1
Harvard Partners Center for Genetics and Genomics, Cambridge, MA, USA. kenneth.aprker@virgininstruments.com

Abstract

We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.

PMID:
19382779
DOI:
10.1021/pr800873q
[Indexed for MEDLINE]
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