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Methods Mol Biol. 2009;519:145-69. doi: 10.1007/978-1-59745-281-6_10.

Proteomic analysis of Caenorhabditis elegans.

Author information

1
Yonsei Proteome Research Center, Biomedical Proteome Research Center and Department of Biochemistry, Yonsei University, 134 Shinchon-dong, Sudamoon-Ku, Seoul, 120-749, Korea.

Abstract

Proteomic studies of the free-living nematode Caenorhabditis elegans have recently received great attention because this animal model is a useful platform for the in vivo study of various biological problems relevant to human disease. In general, proteomic analysis is carried out in order to address a specific question with respect to differential changes in proteome expression under certain perturbed conditions. In this chapter, we focus on gel-based proteomic analysis of C. elegans subjected to two specific stress conditions during development: induction of the dauer state for whole body protein expression and a temperature shift for egg protein expression. Utilizing these differently perturbed C. elegans protein samples, two-dimensional electrophoresis and differential in-gel electrophoresis methods have led to the discovery of remarkable aspects of the worm's biology. We also provide numerous details about the technical points and protocols necessary for successful experimentation.

PMID:
19381582
DOI:
10.1007/978-1-59745-281-6_10
[Indexed for MEDLINE]

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