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J Vis Exp. 2009 Apr 20;(26). pii: 1094. doi: 10.3791/1094.

Micro-drive array for chronic in vivo recording: drive fabrication.

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1
Picower Institute for Learning and Memory, Massachusetts Institute of Technology, MA, USA. fkloos@mit.edu

Abstract

Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. Lightweight recording devices carrying a high density array of tetrodes allow for the simultaneous monitoring of the activity of tens to hundreds of individual neurons. Here we describe a protocol for the fabrication of a micro-drive array with twenty one independently movable micro-drives. This device has been used successfully to record from hippocampal and cortical neurons in our lab. We show how to prepare a custom designed, 3-D printed plastic base that will hold the micro-drives. We demonstrate how to construct the individual micro-drives and how to assemble the complete micro-drive array. Further preparation of the drive array for surgical implantation, such as the fabrication of tetrodes, loading of tetrodes into the drive array and gold-plating, is covered in a subsequent video article.

PMID:
19381129
PMCID:
PMC2793172
DOI:
10.3791/1094
[Indexed for MEDLINE]
Free PMC Article
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