Format

Send to

Choose Destination
See comment in PubMed Commons below
Cell Host Microbe. 2009 Apr 23;5(4):386-96. doi: 10.1016/j.chom.2009.03.008.

Clonal conditional mutagenesis in malaria parasites.

Author information

1
Institut Pasteur, Unité de Biologie et Génétique du Paludisme, 28 rue du Dr Roux, Paris cedex 15, Paris 75724, France.

Abstract

We describe here an efficient method for conditional gene inactivation in malaria parasites that uses the Flp/FRT site-specific recombination system of yeast. The method, developed in Plasmodium berghei, consists of inserting FRT sites in the chromosomal locus of interest in a parasite clone expressing the Flp recombinase via a developmental stage-specific promoter. Using promoters active in mosquito midgut sporozoites or salivary gland sporozoites to drive expression of Flp or its thermolabile variant, FlpL, we show that excision of the DNA flanked by FRT sites occurs efficiently at the stage of interest and at undetectable levels in prior stages. We applied this technique to conditionally silence MSP1, a gene essential for merozoite invasion of erythrocytes. Silencing MSP1 in sporozoites impaired subsequent merozoite formation in the liver. Therefore, MSP1 plays a dual role in the parasite life cycle, acting both in liver and erythrocytic parasite stages.

PMID:
19380117
DOI:
10.1016/j.chom.2009.03.008
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center