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Methods Mol Biol. 2009;537:243-61. doi: 10.1007/978-1-59745-251-9_12.

Computational gene annotation in new genome assemblies using GeneID.

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Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Spain.


The sequence of many eukaryotic genomes is nowadays available from a personal computer to any researcher in the world-wide scientific community. However, the sequences are worthless without the adequate annotation of the biological meaningful elements. The annotation of the genes, in particular, is a challenging task that can not be tackled without the aid of specific bioinformatics tools. We present in this chapter a simple protocol mainly based on the combination of the program GeneID and other computational tools to annotate the location of a gene, which was previously annotated in D. melanogaster, in the recently assembled genome of D. yakuba.

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