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Methods Mol Biol. 2009;546:133-43. doi: 10.1007/978-1-60327-977-2_9.

Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

Author information

1
Department of Surgery, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.

Abstract

Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.

PMID:
19378102
DOI:
10.1007/978-1-60327-977-2_9
[Indexed for MEDLINE]

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