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Methods Mol Biol. 2009;524:181-201. doi: 10.1007/978-1-59745-450-6_13.

Epitope mapping using phage display peptide libraries.

Author information

1
Wilex AG, Grillparzerstr. 10, 81675 Munich, Germany.

Abstract

Phage libraries displaying millions of peptides with randomized sequences are extremely useful tools for mapping antibody epitopes. In many cases, antibodies are able to select peptides with reasonable affinity for their combining sites (paratopes) from these libraries. Ideally, consensus motives can be deduced from multiple peptide sequences and matched to areas of the antigen against which the antibody was raised. That way, critical components of the antibody epitope can be defined. This chapter focuses on technical details of epitope mapping employing pre-made filamentous phage peptide display libraries. Examples are given for illustration.

PMID:
19377945
DOI:
10.1007/978-1-59745-450-6_13
[Indexed for MEDLINE]

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