Send to

Choose Destination
See comment in PubMed Commons below
J Smooth Muscle Res. 2009 Feb;45(1):55-62.

Identification of PKC isoforms expressed in human bronchial smooth muscle cell.

Author information

Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, Japan.


The PKC family has been implicated in a wide range of biological response in a number of different cellular systems. Although the functions of PKC have been vigorously studied using pharmacological tools, there is little information about the expression pattern of its isoforms in human bronchial smooth muscle cells (hBSMCs). In the present study, RT-PCR and immunoblot analyses were carried out to detect all the PKC isoforms expressed in cultured normal hBSMCs. The RT-PCR analyses revealed that mRNAs of the known PKC isoforms except for PKCgamma and theta were detected in the hBSMCs. Among them, protein expressions of the isoforms except for PKCeta and mu were also detected by immunoblottings. Thus, the present study clearly demonstrated the expression of multiple PKC isoforms, i.e., PKCalpha, beta, delta, epsilon, zeta and iota in hBSMCs.

[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for J-STAGE, Japan Science and Technology Information Aggregator, Electronic
    Loading ...
    Support Center