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Eukaryot Cell. 2009 Jun;8(6):916-9. doi: 10.1128/EC.00041-09. Epub 2009 Apr 17.

Using morpholinos for gene knockdown in Giardia intestinalis.

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  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

Abstract

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

PMID:
19377039
PMCID:
PMC2698301
DOI:
10.1128/EC.00041-09
[PubMed - indexed for MEDLINE]
Free PMC Article
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