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Diagn Microbiol Infect Dis. 2009 Jul;64(3):344-6. doi: 10.1016/j.diagmicrobio.2009.03.009. Epub 2009 Apr 18.

A simple 3-step algorithm for improved laboratory detection of Clostridium difficile toxin without the need for tissue culture cytotoxicity neutralization assays.

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Kaiser Permanente NW, Department of Pathology, Portland, OR 97230, USA.


This report describes a 3-step algorithm for the detection of Clostridium difficile using an enzyme immunoassay for glutamate dehydrogenase (GDH) antigen, a lateral flow assay for C. difficile toxin, and anaerobic broth culture. This method was found to detect more toxin-containing stool samples than the use of a combination GDH antigen plus toxin A/B testing alone.

[Indexed for MEDLINE]

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