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Trends Biochem Sci. 2009 May;34(5):264-72. doi: 10.1016/j.tibs.2009.01.010. Epub 2009 Apr 15.

Nucleases and helicases take center stage in homologous recombination.

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Department of Microbiology, Columbia University Medical Center, New York, NY 10032, USA.


Homologous recombination (HR)-mediated DNA double-strand break repair maintains genome integrity. Although long-studied, an understanding of two essential steps in this process -- the resection of DNA ends to produce recombinogenic 3' single-stranded DNA tails and the resolution of recombination intermediates -- has remained elusive. Recent findings show an unexpected role for the Sgs1 (BLM) helicase and Dna2 nuclease in end resection, and provide mechanistic insight into the initiation of 5'-3' resection as well as its regulation by the cell cycle and the DNA damage response. Moreover, the identification of a novel Holliday junction resolvase, Yen1 (GEN1), and several helicases that dismantle strand invasion intermediates has increased the repertoire of nucleases and helicases capable of resolving recombination intermediates.

[Indexed for MEDLINE]

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