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Methods Enzymol. 2009;458:575-96. doi: 10.1016/S0076-6879(09)04823-X.

Chapter 23. Cyanobactin ribosomally synthesized peptides--a case of deep metagenome mining.

Author information

1
Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA.

Abstract

Deep metagenome mining is a new method for engineering natural product pathways, focusing on examining symbiotic organisms. The method has been applied to a family of compounds known as cyanobactins, which are ribosomally synthesized peptides produced by cyanobacteria. Often, these cyanobacteria live symbiotically with marine animals, leading to production of natural products in whole animal samples. Here, we focus on methods to identify, clone, and study cyanobactin natural product genes from axenic organisms and metagenomic environments. The application to deep metagenome mining is described, along with other potential targets of this methodology.

PMID:
19374999
PMCID:
PMC2810138
DOI:
10.1016/S0076-6879(09)04823-X
[Indexed for MEDLINE]
Free PMC Article

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