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Anal Biochem. 2009 Jul 15;390(2):203-5. doi: 10.1016/j.ab.2009.04.013. Epub 2009 Apr 15.

Aptamer evolution for array-based diagnostics.

Author information

1
Manchester Interdisciplinary Biocenter, University of Manchester, Manchester M1 7DN, UK. mark.platt@manchester.ac.uk

Abstract

Closed loop aptameric directed evolution, (CLADE) is a technique enabling simultaneous discovery, evolution, and optimization of aptamers. It was previously demonstrated using a fluorescent protein, and here we extend its applicability with the generation of surface-bound aptamers for targets containing no natural fluorescence. Starting from a random population, in four generations CLADE produced a new aptamer to thrombin with high specificity and affinity. The best aptameric sequence was void of the set of four guanine repeats typifying thrombin aptamers and, thus, highlights the benefits of evolution performed in an environment closely mimicking the final diagnostic application.

PMID:
19374883
DOI:
10.1016/j.ab.2009.04.013
[Indexed for MEDLINE]

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