Format

Send to

Choose Destination
See comment in PubMed Commons below
BMC Genomics. 2009 Apr 16;10:161. doi: 10.1186/1471-2164-10-161.

Estimating accuracy of RNA-Seq and microarrays with proteomics.

Author information

1
Key lab of Systems Biology, Shanghai Institutes for Biological Sciences, China Academy of Sciences, Shanghai, 200031, PR China. xfu@sibs.ac.cn

Abstract

BACKGROUND:

Microarrays revolutionized biological research by enabling gene expression comparisons on a transcriptome-wide scale. Microarrays, however, do not estimate absolute expression level accurately. At present, high throughput sequencing is emerging as an alternative methodology for transcriptome studies. Although free of many limitations imposed by microarray design, its potential to estimate absolute transcript levels is unknown.

RESULTS:

In this study, we evaluate relative accuracy of microarrays and transcriptome sequencing (RNA-Seq) using third methodology: proteomics. We find that RNA-Seq provides a better estimate of absolute expression levels.

CONCLUSION:

Our result shows that in terms of overall technical performance, RNA-Seq is the technique of choice for studies that require accurate estimation of absolute transcript levels.

PMID:
19371429
PMCID:
PMC2676304
DOI:
10.1186/1471-2164-10-161
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Support Center