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Langmuir. 2009 Jun 2;25(11):6307-11. doi: 10.1021/la804308r.

Characterization of the boundary zone of a cast protein drop: fibroin beta-sheet and nanofibril formation.

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1
European Synchrotron Radiation Facility, B.P. 220, F-38043 Grenoble Cedex, France.

Abstract

We have studied a cast fibroin drop with grazing incidence small-angle X-ray scattering, imaging, and spectroscopy techniques. Optical microscopy shows that the dried drop forms a boundary zone. Grazing-incidence small-angle X-ray scattering performed with a synchrotron radiation microbeam in the boundary zone suggests the formation of nanometer-sized domains with one-dimensional paracrystalline order. Atomic force microscopy and scanning electron microscopy support a nanofibrillar morphology. MicroRaman spectroscopy shows the formation of beta-sheet secondary structure in the boundary zone, which is attributed to a shearing effect due to the retraction of the drop boundary upon evaporation.

PMID:
19368340
DOI:
10.1021/la804308r
[Indexed for MEDLINE]
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