Mycobacterium tuberculosis PtkA is a novel protein tyrosine kinase whose substrate is PtpA

Horacio Bach; Dennis Wong; Yossef Av-Gay

doi:10.1042/BJ20090478

Mycobacterium tuberculosis PtkA is a novel protein tyrosine kinase whose substrate is PtpA

Biochem J. 2009 May 13;420(2):155-60. doi: 10.1042/BJ20090478.

Authors

Horacio Bach¹, Dennis Wong, Yossef Av-Gay

Affiliation

¹ Department of Medicine, University of British Columbia, Vancouver, Canada.

PMID: 19366344
DOI: 10.1042/BJ20090478

Abstract

In Mycobacterium tuberculosis, signal transduction is mediated by 11 serine/threonine kinases, but no tyrosine kinases have been identified thus far. The protein encoded by the ORF (open reading frame) Rv2232 has been annotated as a member of the HAD (haloacid dehydrogenase-like hydrolase) superfamily, which includes phosphatases, phosphomanno- and phosphogluco-mutases, and haloacid dehydrogenases. In the present paper, we report, on the basis of biochemical and mutational analyses, that the Rv2232-encoded protein, named protein tyrosine kinase A (PtkA) is a bona fide protein tyrosine kinase. The cognate substrate of PtkA is the secreted protein tyrosine phosphatase A (PtpA).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Binding, Competitive
Kinetics
Mutation
Mycobacterium tuberculosis / enzymology*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / metabolism
Phosphorylation
Protein Binding
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism*
Receptor-Like Protein Tyrosine Phosphatases, Class 4 / genetics
Receptor-Like Protein Tyrosine Phosphatases, Class 4 / metabolism*
Substrate Specificity
Tyrosine / metabolism

Substances

Bacterial Proteins
Tyrosine
Protein-Tyrosine Kinases
Receptor-Like Protein Tyrosine Phosphatases, Class 4