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Curr Protoc Protein Sci. 2009 Apr;Chapter 5:Unit 5.23. doi: 10.1002/0471140864.ps0523s56.

Autoinduction of protein expression.

Author information

1
Department of Biochemistry, Biophysics Degree Program, and Center for Eukaryotic Structural Genomics, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Abstract

This unit contains protocols for the use of lactose-derived autoinduction in Escherichia coli. The protocols allow for reproducible expression trials to be undertaken with minimal user intervention. A basic protocol covers production of unlabeled proteins for functional studies. Alternate protocols for selenomethionine labeling for X-ray structural studies, and multi-well plate growth for screening and optimization are also included.

PMID:
19365792
PMCID:
PMC5602607
DOI:
10.1002/0471140864.ps0523s56
[Indexed for MEDLINE]
Free PMC Article

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