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Biomed Microdevices. 2009 Oct;11(5):951-8. doi: 10.1007/s10544-009-9312-x. Epub 2009 Apr 14.

The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts.

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Department of Bioengineering (MC138-78), California Institute of Technology, Pasadena, CA 91125, USA.


The optofluidic microscope (OFM) is a lensless, low-cost and highly compact on-chip device that can enable high-resolution microscopy imaging. The OFM performs imaging by flowing/scanning the target objects across a slanted hole array; by measuring the time-varying light transmission changes through the holes, we can then render images of the target objects at a resolution that is comparable to the holes' size. This paper reports the adaptation of the OFM for imaging Giardia lamblia trophozoites and cysts, a disease-causing parasite species that is commonly found in poor-quality water sources. We also describe our study of the impact of pressure-based flow and DC electrokinetic-based flow in controlling the flow motion of Giardia cysts--rotation-free translation of the parasite is important for good OFM image acquisition. Finally, we report the successful microscopy imaging of both Giardia trophozoites and cysts with an OFM that has a focal plane resolution of 0.8 microns.

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