Format

Send to

Choose Destination
Biochemistry. 2009 May 12;48(18):3819-21. doi: 10.1021/bi9005418.

Cholesterol metabolism increases the metabolic pool of propionate in Mycobacterium tuberculosis.

Author information

1
Department of Chemistry, Stony Brook University, Stony Brook, New York 11794-3400, USA.

Abstract

Mycobacterium tuberculosis can metabolize cholesterol to both acetate and propionate. The mass of isolated phthiocerol dimycoserate, a methyl-branched fatty acylated polyketide, was used as a reporter for intracellular propionate metabolic flux. When M. tuberculosis is grown using cholesterol as the only source of carbon, a 42 amu increase in average phthiocerol dimycoserate molecular weight is observed, consistent with the cellular pool of propionate and, thus, methylmalonyl CoA increasing upon cholesterol metabolism. In contrast, no shift in phthiocerol dimycoserate molecular weight is observed upon supplementation of medium containing glycerol and glucose with cholesterol. We conclude that cholesterol is a significant source of propionate only in the absence of sugar carbon sources.

PMID:
19364125
PMCID:
PMC2771735
DOI:
10.1021/bi9005418
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center