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J Gastroenterol. 2009;44(6):556-61. doi: 10.1007/s00535-009-0037-7. Epub 2009 Apr 11.

Down-regulation of miR-141 in gastric cancer and its involvement in cell growth.

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1
Gastroenterology Laboratory, Clinical Research Institute, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016 Hangzhou, People's Republic of China.

Abstract

PURPOSE:

Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation.

METHODS:

The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay.

RESULTS:

MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells.

CONCLUSIONS:

These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.

PMID:
19363643
DOI:
10.1007/s00535-009-0037-7
[Indexed for MEDLINE]
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